MNFs were seed at a density of 0.5 to 1 × 105 cells per well in a 24-well plate (Nunc) in 500 μl of DMEM + 1% FBS 24 hours before infection with HSV-1 KOS or HSV-2 333 at multiplicity of infection (MOI) of 0.1. Supernatants were harvested 24 hours later, and virus yield was quantified using the plaque titration assay on Vero cells.

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