TAE684, crizotinib (PF-02341066), LDK378 (ceritinib), lorlatinib, dasatinib, and KD025 were purchased from Selleck Chemicals (Selleckchem). LY294002 and U0126 were purchased from Cell Signaling Technology. The stock solutions were prepared in DMSO and stored at −20°C. IGF-1 was purchased from PeproTech, and insulin was purchased from Sigma-Aldrich. The following antibodies were used: rabbit anti–phospho-ALK (Tyr1604), mouse anti-ALK (31F12), rabbit anti-ALK (C26G7), rabbit anti–phospho-Akt (Ser473) and anti-Akt, mouse anti–phospho-ERK1/2 (Thr202/Tyr204), rabbit anti-ERK1/2, rabbit anti–phospho-FoxO3a (Ser253) (D18H8) and anti-FoxO3a (D19A7), rabbit anti-PI3K p110α and anti-PI3K p85, rabbit anti-IRS1, rabbit anti-IRS2 (L1326), mouse anti-phosphotyrosine (P-Tyr-100), rabbit anti–cleaved caspase-3, rabbit anti–phospho–IGF-1Rβ (Tyr1131)/insulin receptor β (Tyr1146), rabbit anti–insulin receptor β (4B8), and rabbit anti–IGF-1Rβ (D23H3) (Cell Signaling Technology); rabbit anti-IRS2 for immunoprecipitation, mouse anti-GAPDH, anti-SHP2 [M163], and anti-Shc (Abcam); mouse anti-Grb2 (BD Biosciences, San Jose, CA); mouse anti-vinculin (Sigma-Aldrich); mouse anti-phosphotyrosine (4G10) (Millipore, Bedford, MA); and goat anti-rabbit horseradish peroxidase (HRP)–conjugated secondary antibody and goat anti-mouse HRP-conjugated secondary antibody (Jackson ImmunoResearch Laboratories). For phosphoproteomics, anti-phosphotyrosine immunoaffinity beads (P-Tyr-100 and P-Tyr-1000) were purchased from Cell Signaling Technology.

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