Fibroblasts were cultured from skin biopsies taken after obtaining informed consent from three individuals without diabetes mellitus and four affected patients carrying mutations in WFS1 gene, as previously described (62). Human embryonic kidney (HEK) 293T cells were cultured in MegaCell Dulbecco’s Modified Eagle’s Medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich) and 20 mM penicillin/streptomycin (Sigma-Aldrich).

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