qRT-PCR analysis was performed as previously described (16, 59). Total RNA was isolated using RNeasy Plus Micro Kit (Qiagen), and cDNA was synthesized using a SuperScript III kit [Invitrogen with oligo(dT)20 primers]. qPCR was carried out with Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher Scientific) on ABI 7900HT system (Applied Biosystems) and QuantStudio 5 (Thermo Fisher Scientific). Gene-specific primer sets were obtained from PrimerBank (https://pga.mgh.harvard.edu/primerbank) (60) or designed by using Primer-BLAST (www.ncbi.nlm.nih.gov/tools/primer-blast). The primer sequences are available in table S1.

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