His6-HipA and His6-HipA7. Plasmid pBAD33::6his hipA was transformed into MG1655 strain. An overnight culture was grown in LB medium containing chloramphenicol (25 μg/ml) and 0.4% glucose, washed, diluted 1000× into 2 liters of LB medium containing chloramphenicol (25 μg/ml), and grown at 37°C. The expression of hipA and hipA7 was induced at OD600nm of 0.4 with 0.2% arabinose for 2 hours. His6-HipA7 was produced from pBAD33::6his hipA7 in 1 liter of LB medium in the same way as His6-HipA.

His6-GltX, His6-RplK, and SeqA-His6. Plasmid pET28a::gltX was transformed into BL21(DE3) strain. An overnight culture was grown in LB medium containing kanamycin (50 μg/ml), diluted 1000× into 250 ml of LB medium with kanamycin, and grown at 30°C. The expression of gltX was induced at OD600nm of 0.6 with 1 mM IPTG for 2 hours. His6-RplK was produced from the pET28a::rplK plasmid the same way as His6-GltX. SeqA-His6 was produced from the pET28a::seqA plasmid in 750 ml of LB medium the same way as His6-GltX. The expression of seqA was induced at OD600nm of 0.5 with 0.2 mM IPTG for 2 hours.

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