It is known that ΔrelA mutant exhibits relaxed phenotype when grown in the presence of single carbon amino acids (SMG) (31). E. coli MG1555, rplK S102A, rplK S102D, and ΔrelA deletion mutant (table S1) were grown in M9 medium with glucose for 24 hours to stationary phase. Aliquots of cells were serially diluted in M9 medium, plated on M9 agar plates supplemented with or without 1 mM amino acids SMG, and grown for 40 hours at 37°C.

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