Multiplex cytokine assay was performed according to our previous publication (93). Briefly, 40 μl of treatment medium was incubated overnight with 40 μl of primary antibodies conjugated with magnetic beads. After incubation with primary antibodies, samples were washed, incubated with a biotinylated antibody and biotin-streptavidin, and then read in a Bio-Plex plate reader. Standards were obtained from PeproTech. For welder serum cytokine analysis, the Bio-Plex Pro Human Cytokine 27-plex Assay (M500KCAF0Y, Bio-Rad) was performed according to the manufacturer’s protocol.

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。