Griess assay was performed as previously described in our publication (93). Microglial cells (50,000 to 100,000) were plated in 96-well plates and treated in 2% FBS-containing medium. After treatment, 50 μl of medium was collected and incubated with 50 μl of Griess reagent for 10 min. A plate reader was used to read the absorbance at 540 nm. Sodium nitrite solution was used for making standard curve.

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