Raw mass spectral data files were searched against the SwissProt database containing Homo sapiens protein sequences (20,199 sequences), using Mascot version 2.4 (Matrix Science). TMT reporter quantification was extracted and isotope-corrected using Proteome Discoverer software (Thermo) and was further normalized on the basis of median relative protein quantification ratios obtained from total protein expression analysis. For each peptide, relative quantification was represented as a ratio between ion intensities for experimental conditions and normalization channel.

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