RNA was extracted using miRNeasy Mini Kit (QIAGEN), according to the manufacturer’s protocol. The quantification and quality analysis of RNA were performed on a Bioanalyzer 2100 (Agilent) using RNA 6000 Nano Kit (Agilent). Synthesis of complementary DNA (cDNA) and biotinylated complementary RNA (cRNA) was performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), according to the manufacturer’s protocol using 500 ng of total RNA. Quality assessment and quantification of cRNAs were performed with Agilent RNA kits on Bioanalyzer 2100. Hybridization of cRNAs (750 ng) was carried out using Illumina Human 48,000 gene chips (HumanHT-12 v4 BeadChip). Array washing was performed using Illumina High-Temp Wash Buffer for 10 min at 55°C, followed by staining using streptavidin-Cy3 dyes (Amersham Biosciences). Probe intensity data were obtained using the Illumina GenomeStudio software (GenomeStudio v2011.1). Raw data were normalized with genome studio, according to the cubic spline normalization.

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