Student’s t test was used to select genes with differential expression between MDA-MB231–mock– and MDA-MB231–Sema4C–overexpressing cells (P < 0.05; absolute log2 ratio, >1). This resulted in 418 unique genes being selected. Monte Carlo analysis calculated on all the possible permutation of samples evaluated an FDR of <0.05. Gene expression clustering was performed using Gene Expression Data Analysis Studio (GEDAS) (56).

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