Equal numbers of exponentially growing WT(JX333) or Δpka1(JX384) cells transformed with pRep41pkc(vector), pRep41Pka1pkc, pRep41Pka1C358Spkc, or pRep41Pka1C358Apkc grown in Edinburgh mimimal medium (EMM) supplemented with adenine (100 mg/liter) and thiamine (20 μg/ml) (EMMAT) were serially diluted (10-fold dilutions then spotted onto an EMMAT agar plate or EMMAT agar plates containing 1 M KCl). Plates were incubated at 30°C for 2 to 5 days and imaged at 24-hour intervals until comparable levels of growth were observed for the WT cells on each plate.