RNA was extracted with QIAzol (QIAGEN) and precipitated with isopropanol. cDNA was generated from 3.5 μg of total RNA template using oligo(dT)20 and SuperScript III (Invitrogen). Analysis of relative RNA expression was performed by quantitative polymerase chain reaction in real time. The relative RNA levels of indicated genes were normalized to the average RNA level of two control genes rRNA18S and FAM50A in the same samples. Data presented were obtained from four independent experiments.

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