Human ovarian cancer cell lines, CAOV3 and SKOV3, were ordered from American Type Culture Collection (Manassas, VA, USA). The normal cell line used in this study was human ovarian surface epithelial cell line (HOSEpiC) obtained from ScienCell Research Laboratories (San Diego, CA). SKOV3 cells were grown in McCoy's 5A (Invitrogen, Carlsbad, CA) medium. Moreover, CAOV3 and HOSEpiC cells were grown in Dulbecco's Modified Eagles Medium. All above mediums were supplemented with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CAUSA), 100 U/mL penicillin, and 100 μg/mL streptomycin and incubated in a humidified atmosphere at 37 °C with 5% CO2.Moreover, cells were cultured in antibiotic-free medium before transfection.