Cell viability was measured by using a CellTiter 96 AQueous one solution cell proliferation assay kit (Promega) following the manufacturer's protocol. Briefly, MCF7 and T47D cells were transfected with si-circPGR or pCD2.1-circPGR and maintained in culture medium for different time points followed by cell proliferation assay. To measure cell viability, 20 µl of CellTiter 96 AQueous one solution reagent was added per 100 µl of culture medium, and the culture plates were incubated at 37 °C for 1 h in a humidified, 5% CO2 atmosphere incubator. The reaction was stopped by adding 25 µl of 10% SDS. Data was recorded at wavelength 490 nm using a Thermo Multiskan MK3 Microplate Reader.

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