One gram each of pulverised samples of the plant was mixed with 20 ml of petroleum ether. This was poured out into a test tube and then left to evaporate to dryness. Later, 0.2 ml of chloroform–acetic anhydride (1:1, v/v) was added to the residue and 2 ml of TCA-chloroform (1:1, v/v) was added to the solution obtained. Vitamin A standard (retinol) was prepared using the same procedure for the samples. Final concentration of 1 mg/ml was made for both standard and plant extracts and serial dilution of the concentration was made given concentration levels 1, 0.8, 0.6, 0.4 and 0.2 mg/ml. The absorbance of the resulting solution and vitamin A standard was measured at 620 nm. The concentration of vitamin A in the sample was deduced from the standard curve. The vitamin A content is measured (in milligrams) as retinol activity equivalence/100 g (RAE/100 g DW).

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