Vitamin E content was determined by mixing 1 g each of the samples with 20 ml ethanol. The mixture was filtered and to 1 ml of the filtrate, 1 ml each of 0.5% α-α-dipyridine and 0.2% ferric chloride in ethanol was added. The solution was made up to 5 ml with distilled water. Vitamin E standard (tocopherol) solution was also prepared using the same procedure as samples. The final concentration of 1 mg/ml was made for both standard and samples. Serial dilution of the concentration was later done giving concentration levels 1, 0.8, 0.6, 0.4 and 0.2 mg/ml. The absorbance of both samples solution and Vitamin E standard solution was read. Vitamin E content in milligrams alpha tocopherol/100 g DW was deduced from the standard curve.

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