Phytate content was determined as described by52 with modification. Sample of I g of the sample was put into a flask, 50 ml of 2% HCL was added and soaked for 3 h and then filtered. The filtrate (25 ml) was poured into another 250 ml conical flask and thiocyanate solution was added as an indicator. To attain the normal acidity, 53.5 ml of distilled water was added. The solution was then titrated against the standard Iron 111 chloride solution (0.00195 g of iron per ml) until a brownish yellow colour persisted for 5 min.

Phytate was calculated as follows: