The method of53 was adopted to determine the alkaloid content in the samples. Acetic acid (10%) was prepared in ethanol and 1 g of the sample was soaked in 50 ml of 10% acetic acid for 4 h. The content was later filtered using a vacuum pump and concentrated in a water bath to a small quantity. Concentrated Ammonium hydroxide was added dropwise until precipitate (until the cloudy fumes cease). The solution was allowed to cool down, then filtered using the pre-weighed filter paper. The filter paper retained the precipitate/ residue. The precipitate on the filter paper was oven-dried and weighed.

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。