The method of53 was adopted to determine the alkaloid content in the samples. Acetic acid (10%) was prepared in ethanol and 1 g of the sample was soaked in 50 ml of 10% acetic acid for 4 h. The content was later filtered using a vacuum pump and concentrated in a water bath to a small quantity. Concentrated Ammonium hydroxide was added dropwise until precipitate (until the cloudy fumes cease). The solution was allowed to cool down, then filtered using the pre-weighed filter paper. The filter paper retained the precipitate/ residue. The precipitate on the filter paper was oven-dried and weighed.
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