Attenuance (D600 nm) measurements were taken every 10 min from selective or non-selective NM minimal media subcultures grown with IPTG (100 µM) and continuous shaking for 18 h at 37 °C in a Synergy H1 microplate reader (BioTek). For subculturing, 96-well SensoPlate microplates with a clear, flat bottom (Greiner Bio-One) were used. Each 200 µl subculture was generated by dilution (1:100) from independent post-exponential rich media cultures; these 1 mL rich cultures were inoculated and grown in 96-well assay blocks as described above for GFP fluorescence assays.

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。