Fresh culture medium was replaced when the density of Caski-FRA1/Caski-empty vector and Hela-FRA1/Hela-empty vector cells in the 6-well plate reached 50-60%, and LPS (50 ug/ml) was added to the cells for 24h at 37°C. The extracellular medium was collected and the glucose levels in the culture medium were measured according to the protocol as defined in the commercial Glucose Assay Kit (Jiancheng, Nanjing, China). The absorbance at 505 nm of the Glucose level was measured using a SoftMax Pro 6.4 microplate reader (Beckman Coulter, USA).

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