RAW264.7 cells and HEK293 cells were treated with Sb-EE at the indicated concentrations for 24 h. Then, 10 μL of MTT solution was added and incubated for 3 h and the reaction was stopped using stopping solution (15% SDS), as reported previously (Hong et al. 2018). The samples were then incubated overnight. The absorbance of MTT formazan was measured at a wavelength of 540 nm.

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