RAW264.7 cells were pre-treated with Sb-EE for 30 min and then stimulated by LPS (1 µg/mL) for 6 h. Total RNA was isolated using TRIzol reagent according to the manufacturer’s instructions. Semi-quantitative PCR was performed as reported previously (Baek et al. 2016; Lee et al. 2019). The sequences of primers used in this study are listed in Table 1.

Sequences of PCR primers used in this study.

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