Experiments were performed on male C57Bl/6 mice (20–23 g) obtained from the Animal Facilities of Instituto Gonçalo Moniz/FIOCRUZ (Brazil). Mice were housed under a 12 : 12 h dark-light cycle. Animals had access to water and food ad libitum and were housed in temperature-controlled rooms (22–25°C). All behavioral tests were performed between 8:00 a.m. and 5:00 p.m., and animals were only tested once. Animal care and handling procedures were in accordance with the National Institutes of Health guide for the care and use of laboratory animals (NIH, 8023) and the Institutional Animal Care and Use Committee FIOCRUZ (CPqGM 025/2011). Every effort was made to minimize the number of animals used and to avoid any unnecessary discomfort.

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