Hormone levels in mice were monitored dynamically before and after ovariectomy and ovary transplantation by ELISA. Venous blood samples were collected from the eye socket veins in mice. Serum was extracted at three time points: before castration, after castration, and 30 days after transplantation. Serum was separated and stored at −80°C until use. Repeated freeze/thaw cycles were avoided. Serum FSH and AMH levels were measured by using an ELISA kit for mice following the manufacturer’s instructions (Westang, Shanghai, China).

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