Nicotine with a purity of ≥99% and cotinine of 98% purity used as standards in the HPLC method. Trichloroacetic acid (TCA), Thiobarbituric acid (TBA), Naphthalene ethylene diamine dihydrochloride (NED), Methanol, Dichloromethane (DCM), Diethyl ether (DEE), Acetonitrile (ACN), Sodium n-Heptane sulphonic acid, 5, 5′-Dithiobis, 2-nitrobenzoic acid (DTNB), α, α, Dipyridyl, Ninhydrin-buffer reagent, Chloroform, Phenol, Folin ciocalteau reagent, Sodium dodecyl sulfate, and Potassium dihydrogen phosphate (KH2PO4) were purchased from Sigma Aldrich, Bangalore.

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