Healthy adult male SD rats, which weighed 190–230 g supplied by the Experimental Animal Center of Zhengzhou University (Zhengzhou, China), were housed in a standard controlled room (22 ± 1°C) with relative humidity of 60 ± 10% with a 12 h light-dark cycle where light periods were from 6:00–18:00. Rats were raised according to clean grade standards and did not have disease or other adverse symptoms. The Chinese Animal Protection Law was strictly adhered to during the experiment.

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