Histopathological changes in liver tissues with AHF were examined by H&E staining according to our laboratory methods previously described13. Briefly, approximately 5×5×3-mm cuboids from the right lobe of the liver were routinely fixed, washed, dehydrated, cleared and embedded in paraffin. Afterwards, liver sections (4 μm) were stained with H&E (Beyotime Institute of Biotechnology) according to the manufacturer’s instructions. The liver sections were dehydrated with gradient ethanol, cleared in xylene and sealed with neutral gum, and then histological assessment was performed under a microscope.

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。