Synthetic cel-miR-39, miR-34a-5p mimic, and miR-34a-3p mimic (Applied Biosystem) were serially diluted to final concentrations of 300 nM, 30 nM, 3 nM, 0.3 nM, 0.03 nM, 3 pM, 0.3 pM, 0.03 pM, 3 fM, and 0.3 fM. miR-34a-5p, miR-34a-3p, and cel-miR-39 serial dilutions were reverse-transcribed and assayed using real-time PCR analysis concurrently with RNA extracted from tumor samples and normal tissue. 2.5 μL of exogenous cel-miR-39 at a concentration of 200 amol/μl was introduced as an exogenous normalizer before RNA extraction in all samples. Standard curves for miR-34a-5p and miR-34a-3p and cel-miR-39 were included on each plate of the TaqMan microRNA assays to convert the Ct values of each sample into the corresponding number of microRNA copies. The absolute quantification result of miR-34a-5p and miR-34a-3p was obtained by normalization to cel-miR-39.

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。