Western blots were performed using the following established laboratory protocols. Cells were lysed in RIPA buffer and protein was quantified using a Bradford assay (Thermofisher). Proteins were separated using 10% SDS-PAGE electrophoresis and transferred onto polyvinylidene difluoride (PVDF) membranes. The membranes were blocked with 5% nonfat milk and incubated at 4 °C overnight with MET, Vimentin, CDH1, GAPDH, and Actin antibodies (1:1000). The membranes were then re-incubated with anti-rabbit (1:5000) secondary peroxidase-labeled antibodies at room temperature for 2 h. The blots were visualized with ECL Plus reagent (Bio-Rad).

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