RNA from cells, exosomes, and plasma was isolated using a QIAzol Lysis reagent and total RNA was isolated using Direct-zol™ RNA MiniPrep isolation kit (Zymo Research). 100 μL of exosome suspension or plasma from plasma of patients with HNSCC or healthy subjects were mixed with 300 μL QIAzol lysis buffer, and the mixture was processed according to the standard protocol. Quantity and quality of the RNA were determined by NanoDrop 1000 (260/280 and 260/230 ratios).

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。