Quantitative Reverse Transcription PCR (qRT-PCR) was used to determine the expression levels of mRNAs. For mRNA analyses, cDNA was transcribed from 1 μg of total RNA utilizing iScript™ cDNA synthesis kit (Bio-Rad). Real-time quantitative PCR was performed with iTaq Universal SYBR Green Supermix (Bio-Rad). The primer sequences were as follows: human MET (forward), 5′-GAG GCA GTG CAG CAT GTA GT-3′, human MET (reverse), 5′-GAT GAT TCC CTC GGT CAG AA-3′, GAPDH (forward), 5′-TCA GTG GTG GAC CTG ACC TG-3′, GAPDH (reverse), 5′-TGC TGT AGC CAA ATT CGT TG-3′. mouse MET (forward), 5′-GACTTCAGCCATCCCAATGT-3′, mouse MET (reverse), 5′-GGTGAACTTCTGCGTTTGC-3′. human Vimentin (forward), 5′-TGTCCAAATCGATGTGGATGTTTC-3′, human Vimentin (reverse), 5′-TTGTACCATTCTTCTGCCTCCTG-3′. human CDH1 (forward), 5′-GCCTCCTGAAAAGAGAGTGGAAG-3′, human CDH1(reverse), 5′-TGGCAGTGTCTCTCCAAATCCG-3′, mouse Vimentin (forward) 5′-CCCTCACCTGTGAAGTGGAT-3′, mouse Vimentin (reverse) 5′-TCCAGCAGCTTCCTGTAGGT − 3′. mRNA relative levels were calculated using the ΔΔCt method. The relative expression level of each mRNA was presented by 2–ΔΔCt.

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