After the paraffin-embedded tumor tissue was sectioned, IHC staining for Ki67, E-Cadherin, N-Cadherin, and Vimentin was performed to assess the levels of the proteins according to the experimental instructions. Finally, the slides were scanned with the Leica slide scanning microscope imaging system. The result of Ki67 was assessed by the percentage of positive staining cells. The staining of epithelial-mesenchymal transition (EMT) markers were evaluated by IHC scores. The scoring is based on staining intensity: no staining scored 0, weakly staining scored 1, moderately staining scored 2, and strongly staining scored 3. The final score was calculated using the following percentage score × staining intensity score.

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