Data of the study were shown as means ± SD, and at least three independent replicates were conducted. The differences were analyzed by Student’s t-test and analysis of variance using GraphPad Prism 7.0 (San Diego, CA, USA) and SPSS software 24.0 (SPSS Inc., Chicago, IL, USA). Statistical significance was evaluated via the Kaplan-Meier method, the log-rank test, and Pearson’s correlation analysis. p < 0.05 was deemed to indicate statistical significance.

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