The threshold for SARS-CoV-2 positivity was set on the presence of more than 25 viral template copies per milliliter, which was calculated based on a SARS-CoV-2 dilution series of SCV2QC (Qnostics). The cut-off CT-values were determined for each platform separately. Results of samples with CT-values representing ≤25 viral copies per milliliter, but with a visible PCR signal, crossing the threshold, were assigned as ‘borderline’ test results. Samples were considered as being negative if no amplification occurred.

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