CRC cell lines used in the present study were bought from American Type Culture Collection (ATCC, Manassas, VA, USA): HCT116, SW480, SW620, and CaCo-2. Normal enterocyte NCM460 was purchased from QCHENG BIO (Shanghai, China). Cells were cultivated in a humid environment containing 5% CO2 at 37 °C using the homogeneous mixture by Dulbecco’s modified eagle medium (DMEM; Gibco, Carlsbad, CA, USA), 10% fetal bovine serum (FBS; Gibco), and 1% penicillin-streptomycin solution (Gibco).

Short hairpin RNA (shRNA) targeting circ_0082182 (sh-circ) and negative control (sh-NC) vectors, miR-411/miR-1205 mimic (miR-411 and miR-1205) and miRNA mimic NC (miR-NC), miR-411/miR-1205 inhibitor (anti-miR-411 and anti-miR-1205), and miRNA inhibitor NC (anti-NC) were all acquired from GenePharma (Shanghai, China). HCT116 and CaCo-2 cells were transfected with the above products using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, Carlsbad, CA, USA), referring to the manufacturer’s specification.

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