The workload and the VO2 of the mice at fixed-speed running test until fatigue on treadmill was evaluated (LE400, Panlab, Harvard Apparatus, Cornella, Spain) pre (at least 48 h after incremental-speed running test until fatigue) and post 4 weeks aerobic training (at least 48 h after the last training session). The intensity of this test corresponded to 80% of the Speak achieved at incremental-speed running test until fatigue and the treadmill slope adopted was also 5°. The workload attained in the fixed-speed running test was used as a reference for the load prescription in the exercise sessions throughout the aerobic training.