MG20, an experimental strain of Lotus japonicus, was obtained from LegumeBase in the National BioResource Project (https://www.legumebase.brc.miyazaki-u.ac.jp/). Isotopically labelled internal standards including [2H2]-gibberellin A4 (GA4), [2H6]-(+)-cis,trans-abscisic acid (ABA), [2H3]-brassinolide (BL), [2H4]-salicylic acid (SA), [15N4]-trans-zeatin (tZ), [15N4]-cis-zeatin (cZ), [2H6]-(±)-jasmonic acid (JA), and [2H5]-indole-3-acetic acid (IAA) were purchased from OlChemIm Ltd. (Olomouc, Czech Republic). Gibberellin A4 (GA4) was purchased from Santa Cruz Biotechnology, Inc. (CA, USA). Abscisic acid (ABA), trans-zeatin (tZ), and jasmonic acid (JA) were obtained from Tokyo Kasei Kogyo Co. (Tokyo, Japan). Salicylic acid (SA) and indole-3-acetic acid (IAA) were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). Brassinolide (BL) was from Cayman Chemical (MI, USA). cis-Zeatin (cZ) was from Santa Cruz Biotechnology Inc. Quartz sand was obtained from Tochu Co. (Aichi, Japan). All the other chemicals were the highest-grade commercially available products.

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