Laboratory results were recorded on paper forms by laboratory technicians and then entered to an excel sheet (Microsoft Excel, 2010) along with demographic data. Statistical analysis was performed on SPSS version 23 and openEpi software Version 3.01 (, updated 2013/04/06). Descriptive statistics was used to summarize the data and the diagnostic performance of the Baermann methods was assessed as sensitivity, specificity, Negative Predictive Value (NPV) and Positive Predictive Value (PPV) against the composite reference standard. McNemar test was used to compare the sensitivity, specificity and relative predictive values between the methods and Cohen’s kappa was used to test the agreement of each method with the composite reference standard. P-values <0.05 were considered to be statistically significant.