PRX-IIE (AT3G52960), Glutaredoxin-S12 (GRX-S12; AT2G20270), and sulfiredoxin (SRX; AT1G31170) were cloned into pET28a (Novagen, Darmstadt, Germany). Forward and reverse primer were designed with NdeI and BamHI restriction sites, respectively (Table S1). The variants C121S, C146S, C121S/C146S, S82D, T108E, and T223E of PRX-IIE were generated by site-directed mutagenesis with specific primers (Table S1). The correctness of all constructs was confirmed by DNA sequencing.

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