Lifespans were measured using established protocols (Linford et al., 2013). Normally, six replicate vials (~150 experimental flies) were established for each treatment. Flies were transferred to fresh media every 2‐3 days, at which time dead flies were removed and recorded using the DLife system developed in the Pletcher Laboratory (Linford et al., 2013). Flies were kept in constant temperature (25°C) and humidity (60%) conditions with a 12:12 hr light:dark cycle.
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