The AID system was used essentially as described (Nishimura et al., 2009). Briefly, cells expressed C-terminal fusions of the protein of interest to an auxin responsive protein (IAA7) at the endogenous locus. Cells also expressed TIR1, which is required for auxin-induced degradation. 500 μM IAA (indole-3-acetic acid dissolved in DMSO; Sigma) was added to media to induce degradation of the AID-tagged protein. Auxin was added for 30 min prior to harvesting cells or as is indicated in figure legends.

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。