Flies were raised according to our standard husbandry protocols (see above), and following eclosion, males and females were allowed to mate on SY10 food for 48–72 hr. Eight females and eight males were then sorted into individual vials, which contained either a choice or a fixed diet. Egg counts were taken on days 1, 2, 5, and 8 after sorting. Flies were transferred to fresh SY food 24 hr prior to the counting.

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