All experiments were carried out 3 times. Data analysis was performed using GraphPad Prism 8 (GraphPad Software, Inc.). Student's t-tests were used to determine the difference between the 2 groups for the behavioral data, immunohistochemistry, RT-qPCR, MTS assay and western blot analysis data (PC12 cells induced with gradient 6-OHDA and transfected with FTH1 siRNA). One-way ANOVA followed by post hoc Tukey's multiple comparison tests were used to analyze the difference between groups in the remaining western blot analysis data, cellular iron concentration assays, MMP, cell ROS detection and DLR assay data. Data are presented as the means ± SEM. P<0.05 was considered to indicate a statistically significant difference.