The morphology of the nanoparticles was characterized using a transmission electron microscope (TEM; Tecnai G2 F20 S-TWIN TEM) with negative electron staining of the phosphotungstic acid protein. Furthermore, the size of the nanoparticles was tested by dynamic light scattering using a Brookhaven Zeta PALS analyzer. The ICG concentration of the nanomaterials was determined by the ultraviolet absorption method. Prior to the tests, different ICG standard concentration working solutions were prepared (0, 2.5, 5, 7.5, and 10 μM) to construct a standard curve and measure the content of ICG in PHSA-ICG-TAT and PHSA-ICG. In addition, 5 μM of ICG, PHSA, PHSA-ICG, and PHSA-ICG-TAT were dissolved in 50% ethanol for 200–900 nm ultraviolet spectrum analysis.

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