The 4T1 cells (5×105 cells/well) were seeded in six-well plates and incubated overnight at 37°C in a humidified 5% CO2 atmosphere. ICG, PHSA-ICG, and PHSA-ICG-TAT (10 μM), as well as PBS (Control), were added to the cells, and the solutions were incubated for 24 h. The cells were irradiated with light at a wavelength of 808 nm (power density: 2.0 W/cm2) for 5 min and cultured further for 24 h.

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