When the tumor size reached 2.5×2.5 mm, 30 μL of PHSA-ICG-TAT (equivalent to 15 μM of ICG) was injected into the tail vein of the mice for 24 h. Subsequently, in vivo fluorescence imaging was performed. The heart, liver, spleen, lung, and kidney tissues and tumor tissues of mice were dissected for ex vivo fluorescence imaging. The fluorescence was recorded using the Maestro all-optical imaging system (Caliper Life Sciences, Hopkinton, MA, USA).

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