LncRNA XIST-Mut or lncRNA XIST-Wt were co-transfected with miR-192 mimic or NC into HepG2.2.15 cells for 48 h. In addition, TRIM25-Mut or TRIM25-Wt was transfected with miR-192 mimic or NC into HepG2.2.15 cells for 48 h. Luciferase activity in cells was determined using the dual-luciferase assay kit (Promega) following the manufacturer’s instructions. Renilla luciferase was used as a control reporter for normalization.
Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。