For etanercept studies, 6-month-old FcγRIIb-/- males and WT controls were subcutaneously injected with 16 doses of either PBS or 25 mg/kg etanercept (Enbrel, Wyeth, New Jersy, USA) which is an intermediate dose twice a week for 8 weeks [16]. At the end of the experiment, blood samples were collected, centrifuged at 10,000 rpm for 10 minutes and kept at −80°C for determination of serum proinflammatory cytokines and PTH levels. Left mandibles were fixed in 10% neutral buffered formalin for μCT analysis. Right mandibles were frozen in liquid nitrogen and kept at −80°C for RNA isolation and qPCR.
Copyright and License information: ©2021 Sakunrangsit et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.