Total RNA was extracted from the right mandibles using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s procedure. RNA samples were further purified using an RNeasy Mini kit (Qiagen, Germantown, MD, USA) and the RNA yields were measured using a NanoDrop 1000 (Thermo Fisher Scientific, CA, USA). The cDNA was synthesized from 500 ng of total RNA with SuperScript VILO cDNA synthesis kit (Invitrogen, Carlsbad, CA, USA). The qPCR reaction was performed using the Luna Universal qPCR Master Mix (New England Biolabs) and was conducted at 60°C for 40 cycles using CFX96TM Optics Module (Bio-Rad, CA, USA). Gene expression profiles were normalized to GAPDH. The oligonucleotide primers for qPCR are shown in Supplementary S1 Table.

注意:以上内容是从某篇研究文章中自动提取的,可能无法正确显示。



Q&A
请登录并在线提交您的问题
您的问题将发布在Bio-101网站上。我们会将您的问题发送给本研究方案的作者和具有相关研究经验的Bio-protocol成员。我们将通过您的Bio-protocol帐户绑定邮箱进行消息通知。